Details of Functionality | Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-6ng/mL.The specific activity of recombinant human VEGF165 is approximately 1.7 x 103 U/μg,which is calibrated against recombinant human VEGF165 WHO Standard (NIBSC code: 02/286). |
Source | Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VEGF proteinAla27-Arg191 |
Accession # | NP_001165097 |
N-terminal Sequence | Ala27 |
Structure / Form | Disulfide-linked homodimer |
Protein/Peptide Type | Recombinant Proteins |
Gene | VEGFA |
Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note | <0.01 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">0.01> |
Theoretical MW | 19.2 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
SDS-PAGE | 20-22 kDa, reducing conditions39-42 kDa, non-reducing conditions | |
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