Summary:

Immunogen	KLH-conjugatedhumanCaspase-3syntheticpeptide CRGTELDCGIETD Accession#U26943
Specificity	DetectshumanandmouseCaspase-3cleavedatAsp175.Nocross-reactivitywasdetectedwiththefull-lengthprocaspase-3orothercaspases.
Source	N/A
Isotype	IgG
Clonality	Monoclonal
Host	Rabbit
Gene	CASP3
Innovator"sReward	Testinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase. LearnabouttheInnovator"sReward

Applications/Dilutions:

Dilutions	WesternBlot0.5ug/mL Immunocytochemistry8-25ug/mL
ReviewedApplications	Read4Reviewsrated4.5 using MAB835inthefollowingapplications: WesternBlot Immunofluorescence Immunohistochemistry-Paraffin
Publications	ReadPublicationsusing MAB835inthefollowingapplications: ICC 1publication IF 1publication IHC 4publications IHCFrozen 2publications IHC-Notspecified 1publication IHCFresh 1publication IHCFrozen 3publications IHCParaffin-embedded 2publications IHCparaffinembedded 1publication WB 8publications

Packaging,Storage&Formulations:

Notes:

• Apopain
• apoptosis-relatedcysteineprotease
• CASP3
• CASP-3
• caspase3,apoptosis-relatedcysteinepeptidase
• Caspase3
• Caspase-3
• CPP32
• CPP-32
• CPP32B
• CPP32SREBPcleavageactivity1
• CysteineproteaseCPP32
• EC3.4.22
• EC3.4.22.56
• LICE-1
• PARPcleavageprotease
• procaspase3
• ProteinYama
• SCA-1
• YAMA

AlternateNamesfor53BP1Antibody:

Caspase-3(Cysteine-asparticacidprotease3/Casp3;alsoYama,apopainandCPP32)isa29kDaheterodimerthatbelongstothepeptidaseC14Afamilyofenzymes.Itiswidelyexpressedandconsideredtobethemajorexecutionercaspaseintheapoptoticcascade.Humanprocaspase-3isa32kDa,277aminoacid(aa)proteinandisnormallyaninactivehomodimer.Followingcellstress/activation,procaspase-3undergoesproteolysistogenerateanN-terminal148aap17/17kDasubunit(aa29-175),plusa102aaC-terminalp12/12kDasubunit.Thesesubunitsnoncovalentlyheterodimerize,andassociatewithanotherp17/p12heterodimertoformanactiveenzyme.Thereisonepotentialvariantthatshowsanalternativestartsitenineaaupstreamofthestandardstartsitecoupledwitha21aasubstitutionforaa162-277.Overaa29-175,humanandmousecaspase-3share87%aaidentity.

Background:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.PrimaryAntibodiesareguaranteedfor1yearfromdateofreceipt.

Limitations:

Caspase-3(Cysteine-asparticacidprotease3/Casp3;alsoYama,apopainandCPP32)isa29kDaheterodimerthatbelongstothepeptidaseC14Afamilyofenzymes.Itiswidelyexpressedandconsideredtobethemajorexecutionercaspaseintheapoptoticcascade.Humanprocaspase-3isa32kDa,277aminoacid(aa)proteinandisnormallyaninactivehomodimer.Followingcellstress/activation,procaspase-3undergoesproteolysistogenerateanN-terminal148aap17/17kDasubunit(aa29-175),plusa102aaC-terminalp12/12kDasubunit.Thesesubunitsnoncovalentlyheterodimerize,andassociatewithanotherp17/p12heterodimertoformanactiveenzyme.Thereisonepotentialvariantthatshowsanalternativestartsitenineaaupstreamofthestandardstartsitecoupledwitha21aasubstitutionforaa162-277.Overaa29-175,humanandmousecaspase-3share87%aaidentity.