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℡ 4000-520-616
℡ 4000-520-616
Novus/Recombinant Human IL-4 Protein/50 ug/204-IL-050
产品编号：204-IL-050
市  场 价：¥13580.00
场      地：美国(厂家直采)
产品分类： 蛋白类>酶>激酶>
联系QQ：1570468124
电话号码：4000-520-616
邮      箱： info@ebiomall.com
美  元  价：$679.00
品      牌： Novus
公      司：Novus Biologicals
公司分类：
Novus/Recombinant Human IL-4 Protein/50 ug/204-IL-050
商品介绍

RecombinantHumanIL-4ProteinSummary

AdditionalInformation
ANewrhIL-4isNowAvailable!Thenewproteinismammaliancellexpressed!
DetailsofFunctionality
MeasuredinacellproliferationassayusingTF‑1humanerythroleukemiccells.Kitamura,T.etal.(1989)J.CellPhysiol.140:323.TheED50forthiseffectis0.05-0.2ng/mL.ThespecificactivityofrecombinanthumanIL-4isapproximately2.9x104IU/μg,whichiscalibratedagainsthumanIL-4WHOInternationalStandard(NIBSCcode:88/656).
Source
E.coli-derivedhumanIL-4proteinHis25-Ser153,withanN-terminalMet
Accession#
P05112
N-terminalSequence
Met
Protein/PeptideType
RecombinantProteins
Gene
IL4
Purity
>97%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain.
EndotoxinNote
<0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Applications/Dilutions

TheoreticalMW
15kDa.Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
SDS-PAGE
14kDa,reducingconditions
ReviewedApplications
Read2Reviewsrated5
using204-ILinthefollowingapplication:
  • CellProliferation
Publications
ReadPublicationsusing204-ILinthefollowingapplications:
  • Bioassay
    305publications
  • CellCulture
    9publications
  • ELISA(Standard)
    8publications
  • ExVivo
    1publication

Packaging,Storage&Formulations

Storage
Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
  • 12monthsfromdateofreceipt,-20to-70°Cassupplied.
  • 1month,2to8°Cundersterileconditionsafterreconstitution.
  • 3months,-20to-70°Cundersterileconditionsafterreconstitution.
Buffer
Lyophilizedfroma0.2μmfilteredsolutioninPBSwithBSAasacarrierprotein.
Purity
>97%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain.
ReconstitutionInstructions
Reconstituteat100-200μg/mLinsterilePBScontainingatleast0.1%humanorbovineserumalbumin.

Notes

ThisproductisproducedbyandshipsfromR&DSystems,Inc.,aBio-Technebrand.

AlternateNamesforRecombinantHumanIL-4Protein

  • Bcellgrowthfactor1
  • BCDF
  • B-cellstimulatoryfactor1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cellstimulatoryfactor1
  • interleukin4
  • interleukin-4
  • Lymphocytestimulatoryfactor1
  • MGC79402
  • pitrakinra

Background

Interleukin-4(IL-4),alsoknownasBcell-stimulatoryfactor-1,isamonomeric,approximately13kDa‑18kDaTh2cytokinethatshowspleiotropiceffectsduringimmuneresponses(1‑3).Itisaglycosylatedpolypeptidethatcontainsthreeintrachaindisulfidebridgesandadoptsabundledfouralpha-helixstructure(4).HumanIL-4issynthesizedwitha24aasignalsequence.Alternatesplicinggeneratesanisoformwitha16aainternaldeletion.MaturehumanIL-4shares55%,39%and43%aasequenceidentitywithbovine,mouse,andratIL-4,respectively.Human,mouse,andratIL-4arespecies-specificintheiractivities(5‑7).IL-4exertsitseffectsthroughtworeceptorcomplexes(8,9).ThetypeIreceptor,whichisexpressedonhematopoieticcells,isaheterodimeroftheligandbindingIL-4Ralphaandthecommongammachain(asharedsubunitofthereceptorsforIL-2,-7,-9,-15,and‑21).ThetypeIIreceptoronnonhematopoieticcellsconsistsofIL-4RalphaandIL‑13Ralpha1.ThetypeIIreceptoralsotransducesIL-13mediatedsignals.IL-4isprimarilyexpressedbyTh2-biasedCD4+Tcells,mastcells,basophils,andeosinophils(1,2).Itpromotescellproliferation,survival,andimmunoglobulinclassswitchtoIgG4andIgEinhumanBcells,acquisitionoftheTh2phenotypebynaïveCD4+Tcells,primingandchemotaxisofmastcells,eosinophils,andbasophils,andtheproliferationandactivationofepithelialcells(10‑13).IL-4playsadominantroleinthedevelopmentofallergicinflammationandasthma(12,14).

  1. Benczik,M.andS.L.Gaffen(2004)Immunol.Invest.33:109.
  2. Chomarat,P.andJ.Banchereau(1998)Int.Rev.Immunol.17:1.
  3. Yokota,T.etal.(1986)Proc.Natl.Acad.Sci.83:5894.
  4. Redfield,C.etal.(1991)Biochemistry30:11029.
  5. Ramirez,F.etal.(1988)J.Immunol.Meth.221:141.
  6. Leitenberg,D.andT.L.Feldbush(1988)Cell.Immunol.111:451.
  7. Mosman,T.R.etal.(1987)J.Immunol.138:1813.
  8. Mueller,T.D.etal.(2002)Biochim.Biophys.Acta1592:237.
  9. Nelms,K.etal.(1999)Annu.Rev.Immunol.17:701.
  10. Paludan,S.R.(1998)Scand.J.Immunol.48:459.
  11. Corthay,A.(2006)Scand.J.Immunol.64:93.
  12. Ryan,J.J.etal.(2007)Crit.Rev.Immunol.27:15.
  13. Grone,A.(2002)Vet.Immunol.Immunopathol.88:1.
  14. Rosenberg,H.F.etal.(2007)J.AllergyClin.Immunol.119:1303.

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The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products for the lifesciences. Novus Biologicals is also organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research.

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