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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Novus/LC3B Antibody/NB600-1384
产品编号:NB600-1384
市  场 价:¥7580.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$379.00
品      牌: Novus
公      司:Novus Biologicals
公司分类:
Novus/LC3B Antibody/NB600-1384
商品介绍
Immunogen
Polyclonal LC3B Antibody was made to a synthetic peptide made to the N-terminal region of the human LC3B protein. [Uniprot: Q9GZQ8]
Localization
LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.
Marker
Autophagosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP1LC3B
Purity
Immunogen affinity purified
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Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Electron Microscopy
  • ELISA
  • Flow Cytometry 1:200
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 0.1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:400
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Immunoprecipitation
  • Immunohistochemistry Free-Floating
  • Knockdown Validated
  • Knockout Validated
Application Notes
In Western Blot, bands are seen at ~17 and 19 kDa corresponding to LC3-II and LC3-I. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Electron Microscopy was reported in scientific literature (PMID: 21885071). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 24928515). Use in Immunohistochemistry on both paraffin-embedded and frozen sections reported in scientific literature (PMID: 18259115). Use in immunoprecipitation reported in scientific literature (PMID: 26098573). Use in immunoblotting reported in scientific literature (PMID: 25383539). Use in flow reported in scientific literature (PMID: 27622036). Use in ELISA reported in scientific literature (PMID: 21885071). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
14.688 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Brain Hippocampus Membrane Tissue Lysate (Adult Membrane Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Neuro2a Whole Cell Lysate
Neuro2a Chloroquine Treated / Untreated Cell Lysate
HeLa Chloroquine Treated / Untreated Cell Lysate
Reviewed Applications
Read 18 Reviews rated 4.2
using NB600-1384 in the following applications:
  • Western Blot
  • Immunofluorescence
  • Immunocytochemistry
  • Immunohistochemistry
Publications
ReadPublications using NB600-1384 in the following applications:
  • EM
    2 publications
  • FLOW
    4 publications
  • Flow
    4 publications
  • IB
    1 publication
  • ICC
    1 publication
  • ICC/IF
    50 publications
  • IHC
    14 publications
  • IHC-Fr
    2 publications
  • IHC-FrFl
    1 publication
  • IHC-P
    7 publications
  • IP
    1 publication
  • KD
    1 publication
  • WB
    195 publications
品牌介绍

About

Novus Biologicals

Accelerate Scientific Discovery by Developing Unique Products

The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products for the lifesciences. Novus Biologicals is also organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research.

All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving both niche and emerging markets, Novus Biologicals has built solid partnerships with our customers. Contact us for more information.

Novus Biologcials is part of the Bio-Techne family which includes:

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