Immunogen | This LC3A Antibody was prepared from a synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [Uniprot: Q9H492]. |
Localization | LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes. |
Marker | Autophagosome Marker |
Specificity | This LC3A Antibody detects both LC3A and LC3B. |
Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | MAP1LC3A |
Purity | Immunogen affinity purified |
Innovator"s Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator"s Reward |
Dilutions |
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Application Notes | Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications,post translation cleavages, relative charges, and other experimental factors.In ICC, cytoplasmic staining was observed in HeLa cells.Use in immunocytochemistry (PMID: 21545732). and Immunohistochemistry-Paraffin (PMID: 26571030). reported in scientific literature. Use in Flow cytometry reported in scientific literature ( PMID: 24419333).. Use in ELISA reported in scientific literature (PMID: 20930550).. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 31783118)..Use in Southern blot reported in scientific literature (PMID: 21262964).. Use in immunoblotting reported in scientific literature (PMID: 28253371).. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:33035707). | ||||||||||||
Control |
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