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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Novus/LC3A Antibody/NB100-2331SS
产品编号:NB100-2331SS
市  场 价:¥2980.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$149.00
品      牌: Novus
公      司:Novus Biologicals
公司分类:
Novus/LC3A Antibody/NB100-2331SS
商品介绍
Immunogen
This LC3A Antibody was prepared from a synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [Uniprot: Q9H492].
Localization
LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.
Marker
Autophagosome Marker
Specificity
This LC3A Antibody detects both LC3A and LC3B.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP1LC3A
Purity
Immunogen affinity purified
Innovator"s Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator"s Reward
Dilutions
  • Western Blot
  • Simple Western 1:50
  • Chromatin Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100-1:300
  • Immunohistochemistry 1:200-1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Immunoprecipitation 20 ug / 500 ug of lysate
  • Southern Blot
  • Immunohistochemistry Whole-Mount
Application Notes
Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications,post translation cleavages, relative charges, and other experimental factors.In ICC, cytoplasmic staining was observed in HeLa cells.Use in immunocytochemistry (PMID: 21545732). and Immunohistochemistry-Paraffin (PMID: 26571030). reported in scientific literature. Use in Flow cytometry reported in scientific literature ( PMID: 24419333).. Use in ELISA reported in scientific literature (PMID: 20930550).. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 31783118)..Use in Southern blot reported in scientific literature (PMID: 21262964).. Use in immunoblotting reported in scientific literature (PMID: 28253371).. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:33035707).
Control
LC3B Overexpression Lysate
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Neuro2a Whole Cell Lysate
Neuro2a Chloroquine Treated / Untreated Cell Lysate
HeLa Chloroquine Treated / Untreated Cell Lysate
Reviewed Applications
Read 20 Reviews rated 4.4
using NB100-2331 in the following applications:
  • Western Blot
  • Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Immunohistochemistry
Publications
ReadPublications using NB100-2331 in the following applications:
  • ChIP
    1 publication
  • ELISA
    2 publications
  • FLOW
    1 publication
  • IB
    1 publication
  • ICC/IF
    35 publications
  • IHC
    9 publications
  • IHC-Fr
    6 publications
  • IHC-P
    4 publications
  • IHC-WhMt
    1 publication
  • IP
    3 publications
  • WB
    153 publications
  • WB,IP
    1 publication
品牌介绍

About

Novus Biologicals

Accelerate Scientific Discovery by Developing Unique Products

The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products for the lifesciences. Novus Biologicals is also organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research.

All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving both niche and emerging markets, Novus Biologicals has built solid partnerships with our customers. Contact us for more information.

Novus Biologcials is part of the Bio-Techne family which includes:

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