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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Novus/HIF-1 alpha Antibody/NB100-479SS
产品编号:NB100-479SS
市  场 价:¥2980.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$149.00
品      牌: Novus
公      司:Novus Biologicals
公司分类:
Novus/HIF-1 alpha Antibody/NB100-479SS
商品介绍
Immunogen
This HIF-1 alpha antibody was developed against a fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [Uniprot# Q61221].
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIF1A
Purity
Immunogen affinity purified
Innovator"s Reward
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Dilutions
  • Western Blot
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry 1:10 - 1:1000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 5 - 10 ug/mL
  • Immunohistochemistry-Frozen 5 - 10 ug/mL
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Immunoprecipitation 1:10 - 1:500
  • Immunohistochemistry Whole-Mount
Application Notes
WB recognizes a band in hypoxic samples at ~115 kDa. HIF-1 alpha protein (NBC1-18422) has been successfully used for antibody preadsorption/blocking assay in IHC-P with HIF-1 alpha antibody NB100-479. Flow Cytometry usage reported in scientific literature (PMID 21917971). Use in Immunoblotting reported in scientific literature (PMID 28506759). Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 27148974).
Theoretical MW
93 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Pancreas Whole Tissue Lysate (Adult Whole Normal)
COS-7 Nuclear Hypoxic Induced Cell Lysate
Human Bone Marrow Whole Tissue Lysate (Adult Whole Diabetes)
HeLa CoCl2 treated/Untreated Cell Lysate
HepG2 CoCl2 treated/Untreated Cell Lysate
HeLa Hypoxic / Normoxic Cell Lysate
HepG2 Hypoxic / Normoxic Cell Lysate
HIF-1 alpha Knockout HeLa Cell Lysate
Control Peptide
HIF-1 alpha Recombinant Protein (NBC1-18422)
Reviewed Applications
Read 24 Reviews rated 4.4
using NB100-479 in the following applications:
  • Western Blot
  • Chromatin Immunoprecipitation
  • Immunohistochemistry-Paraffin
Publications
ReadPublications using NB100-479 in the following applications:
  • ChIP
    8 publications
  • FLOW
    2 publications
  • IB
    1 publication
  • ICC/IF
    32 publications
  • IHC
    37 publications
  • IHC-Fr
    6 publications
  • IHC-P
    34 publications
  • IHC-WhMt
    1 publication
  • IP
    6 publications
  • WB
    225 publications
  • WB,ChIP
    1 publication
品牌介绍

About

Novus Biologicals

Accelerate Scientific Discovery by Developing Unique Products

The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products for the lifesciences. Novus Biologicals is also organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research.

All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving both niche and emerging markets, Novus Biologicals has built solid partnerships with our customers. Contact us for more information.

Novus Biologcials is part of the Bio-Techne family which includes:

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