Description | This kit is optimized for intracellular staining of cells in flow cytometric applications. It is designed and optimized to minimize non-specific staining while maximizing signal-to-noise ratio for clear and consistent data. |
Kit Type | Flow Cytometry Kit |
Application Notes | Brefeldin A is an inhibitor of intracellular protein transport. It blocks translocation of proteins from the endoplasmic reticulum to the Golgi apparatus and can enhance detection of some intracellular proteins. Researchers are encouraged to determine whether or not Brefeldin A is needed to assay for the protein of interest. Brefeldin A is light-sensitive, store at 4C in the dark. It should be diluted in culture media (1:1000) just prior to use. Add to cell culture and incubate for 4hr before harvesting cells.Use in Flow Intracellular reported in scientific literature (PMID 24804954). | |
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