Additional Information | Newer 145 aa Version is Available! |
Details of Functionality | Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.The specific activity of Recombinant Human FGF basic/FGF2/bFGF is approximately800IU/μg, which is calibrated against recombinant human FGF basic/FGF2basic WHO International Standard (NIBSC code: 90/712). |
Source | E. coli-derived human FGF basic/FGF2/bFGF proteinPro143-Ser288, with an N-terminal Ala |
Accession # | P09038 |
N-terminal Sequence | Ala-Pro143 |
Protein/Peptide Type | Recombinant Proteins |
Gene | FGF2 |
Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note | <0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">0.10> |
Theoretical MW | 16.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
SDS-PAGE | 17 kDa, reducing conditions | |
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