Summary:

Immunogen	AsyntheticpeptidemadetoaC-terminalsequenceofhumanCarbonicAnhydraseIX(withinresidues400-459)[UniProtQ16790]
Localization	Membrane.
Marker	HypoxiaMarker
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	CA9
Purity	Immunogenaffinitypurified
Innovator"sReward	Testinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase. LearnabouttheInnovator"sReward

Applications/Dilutions:

Dilutions	WesternBlot1:2000 SimpleWestern1:50 ChromatinImmunoprecipitation1:10-1:500 ELISA FlowCytometry GelSuperShiftAssays Immunocytochemistry/Immunofluorescence1:1000 Immunohistochemistry1:1000 Immunohistochemistry-Frozen1:1000 Immunohistochemistry-Paraffin1:1000
ApplicationNotes	ThisCarbonicAnhydraseIXantibodyisusefulforWesternblot,Immunofluorescence/Immunocytochemistry,andImmunohistochemistry.GelSuperShiftAssayswasreportedinscientificliterature.InWesternblotabandisobservedapprox.53kDa.UseinELISAreportedinscientificliterature(PMID19963243).Useinchromatinimmunoprecipitationreportedinmultiplepiecesofscientificliterature. InSimpleWesternonly10-15uLoftherecommendeddilutionisusedperdatapoint.SeparatedbySize-Wes,SallySue/PeggySue. Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
TheoreticalMW	55kDa. Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
PositiveControl	HeLaLysate(NB800-PC1) CarbonicAnhydraseIX/CA9Lysate(NBL1-08619)
ControlPeptide	CarbonicAnhydraseIX/CA9Peptide(NB100-417PEP)
ReviewedApplications	Read3Reviewsrated4.7 using NB100-417inthefollowingapplications: WesternBlot Immunocytochemistry
Publications	ReadPublicationsusing NB100-417inthefollowingapplications: ChIP 2publications ELISA 1publication EMSA 1publication ICC/IF 10publications IHC 44publications IHC-FR 1publication IHC-Fr 4publications IHC-P 41publications WB 18publications

ReactivityNotes:

Human,dog,ratandmouse(PMID22842475).Plantreactivityreportedinscientificliterature(PMID:19278636)

Packaging,Storage&Formulations:

Storage	Storeat-20C.Avoidfreeze-thawcycles.
Buffer	PBS
Preservative	0.025%SodiumAzide
Concentration	1.0mg/ml
Purity	Immunogenaffinitypurified

Notes:

• CA9
• CA-IX
• CAIXcarbonicanhydrase9
• CarbonatedehydrataseIX
• CarbonicAnhydraseIX
• carbonicanhydraseIXpMW1
• carbonicdehydratase
• EC4.2.1.1
• G250
• MembraneantigenMN
• MN
• MNRCC-associatedantigenG250
• P54/58N
• RCC
• RCC-associatedproteinG250
• Renalcellcarcinoma-associatedantigenG250

AlternateNamesfor53BP1Antibody:

CarbonicanhydraseIX(CAIX)isamemberoftheCarbonicanhydrasefamilywhichareenzymesthatassistsrapidinterconversionofcarbondioxideandwaterintocarbonicacid,protons,andbicarbonateions.Theyareabundantinallmammaliantissuesandbecauseoftheirfunctionality,theyhavebecomeanimportantdiagnosticmarkerforvariouscancers,mostnotablyrenalcellcarcinoma(RCC).CarbonicAnhydraseIX(CAIX)isoneofthemosthypoxicallyinducIBLegenesbecauseofitsstABIlityandthelocationoftheexpressedproteinwithinthemembrane.CarbonicanhydraseshaveawidespreadroleinregulatingpHinnormaltissues,byregulatinghydrogenion(H+)flux.ThepHisimportantincelldeathunderhypoxia,thusablockadeofCAIX(CA9)resultsinincreasedcelldeathunderhypoxia.Therefore,CAIXhasbecomeareliablehistochemicalmarkerofhypoxia.

Background:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.PrimaryAntibodiesareguaranteedfor1yearfromdateofreceipt.

Limitations:

CarbonicanhydraseIX(CAIX)isamemberoftheCarbonicanhydrasefamilywhichareenzymesthatassistsrapidinterconversionofcarbondioxideandwaterintocarbonicacid,protons,andbicarbonateions.Theyareabundantinallmammaliantissuesandbecauseoftheirfunctionality,theyhavebecomeanimportantdiagnosticmarkerforvariouscancers,mostnotablyrenalcellcarcinoma(RCC).CarbonicAnhydraseIX(CAIX)isoneofthemosthypoxicallyinduciblegenesbecauseofitsstabilityandthelocationoftheexpressedproteinwithinthemembrane.CarbonicanhydraseshaveawidespreadroleinregulatingpHinnormaltissues,byregulatinghydrogenion(H+)flux.ThepHisimportantincelldeathunderhypoxia,thusablockadeofCAIX(CA9)resultsinincreasedcelldeathunderhypoxia.Therefore,CAIXhasbecomeareliablehistochemicalmarkerofhypoxia.