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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Novus/HeLa CoCl2 treated/Untreated Cell Lysate/NBP2-36450
产品编号:NBP2-36450
市  场 价:¥4980.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$249.00
品      牌: Novus
公      司:Novus Biologicals
公司分类:
Novus/HeLa CoCl2 treated/Untreated Cell Lysate/NBP2-36450
商品介绍
PreparationMethod
HeLa cells are cultured using standard media condition until semi-confluent (70-80%).The cells are then treated with or without 100 uM CoCl2 for 4 hours.The cells are then washed in PBS and lysed into 1X Laemmli sample buffer with BME.The lysate is sonicated and tested by Western blot for reactivity to Hif-1a using a polyclonal anti-Hif-1a antibody (NB100-134).Tubulin reactivity of each lysate is shown as a loading control.
Application Notes
HeLa Whole cell lysate is provided as a Western blot positive control for Hif-1 research. The lysates are provided as a set of treated and untreated samples.Use 10 ul per lane for a standard mini-gel blot (approx. 1 mg/ml).
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Novus Biologicals

Accelerate Scientific Discovery by Developing Unique Products

The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products for the lifesciences. Novus Biologicals is also organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research.

All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving both niche and emerging markets, Novus Biologicals has built solid partnerships with our customers. Contact us for more information.

Novus Biologcials is part of the Bio-Techne family which includes:

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