产品编号：NBP2-49689
市 场 价：¥1980.00
场 地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
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info@ebiomall.com
商品介绍
| Description | NBP2-49689 contains 1 vial each of 0.1ml of HeLa Chloroquine treated and 0.1ml of HeLa untreated cell lysate. This lysate set is useful as a positive and negative controls for targets such as LC3, p62 etc. and the researchers should use anti-Human primary antibodies when working with these lysates. |
| Marker | Autophagy/LC3 Positive Control |
| PreparationMethod | HeLa cells were cultured under standard laboratory conditions until semi-confluent (70-80%).The cells were then treated with or without Chloroquine to 50 uM for 24 hours.The cells were washed in PBS and directly lysed into 1X Laemmli sample buffer containing BME.Each lysate is sonicated and boiled before being tested in Western blot for reactivity to LC3.Tubulin reactivity in each lysate is shown as a loading control. |
| Application Notes | HeLa Chloroquine treated / untreated whole cell lysates are provided as positive and negative control for Western blot analysis in Autophagy research. The lysates are provided as one vial of treated and one vial of untreated samples.Use 10 ul per lane for a standard mini-gel blot (approx. 1 mg/ml). |
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Accelerate Scientific Discovery by Developing Unique Products
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products for the lifesciences. Novus Biologicals is also organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research.
All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving both niche and emerging markets, Novus Biologicals has built solid partnerships with our customers. Contact us for more information.
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