产品编号：NBP2-30114
市 场 价：¥7980.00
场 地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
邮 箱：
info@ebiomall.com
商品介绍
| Background | INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Human Cell Lines IMB-105 is a simple and fast solution for screening proteins from various human cell lines. We"ve streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-BlotT...8;, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop. Show More |
| PreparationMethod | INSTA-Blot͐ Human Cell Lines NBP2-30114 contains denatured proteins from human cell line lysates (See Cell Lysate Preparation) loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot is sold ready-to-use in your western blot protocol. |
| Application Notes | Human cell lines are lysed in 1X lysis buffer (10 mM Tris, pH 8.0,130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the cell lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).Use in Western blot reported in scientific literature (PMID: 24842606) | |
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品牌介绍
Accelerate Scientific Discovery by Developing Unique Products
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products for the lifesciences. Novus Biologicals is also organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research.
All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving both niche and emerging markets, Novus Biologicals has built solid partnerships with our customers. Contact us for more information.
Novus Biologcials is part of the Bio-Techne family which includes:
产品分类
销售排行
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总部地址：
江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125)
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