产品编号：NBP2-30111
市 场 价：¥7980.00
场 地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
邮 箱：
info@ebiomall.com
商品介绍
| Description | Ready-to-use PVDF membrane with 11 mouse tissue lysates (20 ug total protein per lane). |
| Background | INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Mouse Tissues NBP2-30111 is a simple and fast solution for screening proteins from various mouse tissues. We"ve streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot͐,...immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop. Show More |
| PreparationMethod | INSTA-Blot™ Mouse Tissues NBP2-30111contains denatured proteins from mouse lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blottedfor 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried andpackaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol. |
| Application Notes | Tissues are from Balb/c mice. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail(PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20%glycerol, 0.4% B-mercaptoethanol). | |
| Publications |
|
品牌介绍
Accelerate Scientific Discovery by Developing Unique Products
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products for the lifesciences. Novus Biologicals is also organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research.
All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving both niche and emerging markets, Novus Biologicals has built solid partnerships with our customers. Contact us for more information.
Novus Biologcials is part of the Bio-Techne family which includes:
产品分类
销售排行
![Novus/Mouse IgG2a Isotype Control (20102) [Unconjugated] (MAB003)/MAB003/500 ug](images/novusbio/IgG2A_MAB003_Control_19426.jpg)
![Novus/RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set/NBP2-29321-5mg](images/novusbio/RelA-NFkB-p65-[p-Ser529--p-Ser536]-Inhibitor-Peptide-Set-Western-Blot-NBP2-29321-img0015.jpg)
![Novus/Caspase-3 Antibody (269518) [Unconjugated] - Cleaved (Asp175) (MAB835)/MAB835/50 ug](images/novusbio/Caspase-3_MAB835_Western_Blot_6292.jpg)
厂家直采
全球直采 正品优价
正品保障
厂家直发 有线跟踪
正规清关
CIF100%正规报关，提供发票
及时交付
限时必达 不达必赔
总部地址：
江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125)
工作时间：周一至周五9:00~18:00
技术支持：
短信：18915418616
服务热线：
4000-520-616（全国）
☏ 0512-67156496（苏州）
版权信息：
© 2015- 苏州蚂蚁淘生物科技有限公司 版权所有 中国
友情链接：
免责声明：
本站有部分内容来自互联网，如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权，请来电或致函告之，
本网站将在规定时间内给予删除等相关处理。
本网站将在规定时间内给予删除等相关处理。
扫描二维码关注官方微信
获取最新促销及产品信息