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℡ 4000-520-616
Novus/NFkB p50 (NLS) Inhibitor Peptide Set/NBP2-29323-5mg
产品编号：NBP2-29323-5mg
市  场 价：¥16980.00
场      地：美国(厂家直采)
产品分类： 抗体类>一抗>功能性抗体>
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美  元  价：$849.00
品      牌： Novus
公      司：Novus Biologicals
公司分类：
Novus/NFkB p50 (NLS) Inhibitor Peptide Set/NBP2-29323-5mg
商品介绍
Immunogen
Functions as a p50 decoy by blocking the intracellular recognition mechanism of p50 NLS.
Specificity
The p50 NLS inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.The control peptide consists of only the PTD sequence
PreparationMethod
Preparation of 5 mM Stock SolutionsPBS* is added directly to the vials to prepare the stock solutions.Note: Bring the solution to room temperature and quick spin the tubes before opening the caps. p50 (NLS)Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKVQRKRQKLMAdd 57 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing. Control Peptide: 1 mg of DRQIKIWFQNRRMKWKKAdd 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.Recipe for 1X PBS:1. Dissolve the following in 800ml distilled H2O. - 8g of NaCl- 0.2g of KCl- 1.44g of Na2HPO4- 0.24g of KH2PO42. Adjust pH to 7.5 with HCl.3. Adjust volume to 1L with additional distilled H2O.4. Sterilize by autoclaving
Content
NF-kB p50 (NLS) Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKVQRKRQKLM (p50 NLS sequence: VQRKRQKLM). Molecular weight: 3529. Antennapedia Control peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
Gene
NFKB1
Application Notes
Inhibition of NF-kB p50(NLS) activity. The inhibitor peptide is designed to block p50 nuclear translocation. Optimal peptide concentrations and incubation times vary between model systems and should be determined empirically by users. A 100 uM final concentration may be a useful starting point. Please refer to Lin et al (1995) for additional information about how the inhibitor peptide has been used to block p50 nuclear translocation. Use in Functional inhibition reported in scientific literature (PMID 24384074)
Publications
ReadPublications using NBP2-29323 in the following applications:
  • B/N
    1 publication
  • Func-INH
    1 publication
品牌介绍

About

Novus Biologicals

Accelerate Scientific Discovery by Developing Unique Products

The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products for the lifesciences. Novus Biologicals is also organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research.

All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving both niche and emerging markets, Novus Biologicals has built solid partnerships with our customers. Contact us for more information.

Novus Biologcials is part of the Bio-Techne family which includes:

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