产品编号：NBP2-26506-5mg
市 场 价：¥16980.00
场 地：美国(厂家直采)
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商品介绍
| Immunogen | Functions as a TRAF6 decoy by binding to the T6DP motif of RANK, thereby preventing binding of RANK to TRAF6. |
| Specificity | The TRAF6 inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable. The control peptide consists of only the PTD sequence. |
| PreparationMethod | Preparation of 5 mM Stock SolutionsPBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.TRAF6 Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRKIPTEDEYAdd 57.7 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.Control Peptide: 1 mg of DRQIKIWFQNRRMKWKKAdd 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing. *Recipe for 1X PBS:1. Dissolve the following in 800ml distilled H2O.- 8g of NaCl- 0.2g of KCl- 1.44g of Na2HPO4- 0.24g of KH2PO42. Adjust pH to 7.5 with HCl.3. Adjust volume to 1L with additional distilled H2O.4. Sterilize by autoclaving |
| Content | TRAF6 Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKRKIPTEDEY (TRAF6 binding sequence: RKIPTEDEY). Molecular weight: 3494.Antennapedia Control peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361. |
| Gene | TRAF6 |
| Application Notes | Inhibition of RANKL mediated osteoclast differentiation.Researchers can study the effect of TRAF6 inhibitor peptide using a variety of methods, such as EMSA, NF-kB/p65 ELISA,IkBa phosphorylation ELISA, osteoclast differentiation assay. The Osteoclast formation assay protocol is a guideline which may need to be optimized for different cell types. Use in In vitro assay and blocking / neutralizing reported in scientific literature (PMID 24829345) | |
| Publications |
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品牌介绍
Accelerate Scientific Discovery by Developing Unique Products
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products for the lifesciences. Novus Biologicals is also organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research.
All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving both niche and emerging markets, Novus Biologicals has built solid partnerships with our customers. Contact us for more information.
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